Immunodiagnosis of bovine fascioliasis by enzyme-linked immunosorbent assay and immunoprecipitation methods.

@article{Hillyer1985ImmunodiagnosisOB,
  title={Immunodiagnosis of bovine fascioliasis by enzyme-linked immunosorbent assay and immunoprecipitation methods.},
  author={George V. Hillyer and Z Sanchez and Deborah de Leon},
  journal={The Journal of parasitology},
  year={1985},
  volume={71 4},
  pages={449-54}
}
Calves were infected with 1,000 Fasciola hepatica metacercariae and the antibodies which developed were monitored by gel diffusion, counterelectrophoresis (CEP) and the enzyme-linked immunosorbent assay (ELISA) using crude or tegument antigen preparations of the adult fluke. Serologic diagnosis was positive as early as 2-4 wk of infection by gel diffusion, by 4 wk by CEP, and by 6-8 wk by ELISA, whereas parasitologic diagnosis via the detection of fluke eggs in the feces was possible only after… CONTINUE READING