Immunodiagnosis of Strongyloides stercoralis infection: a method for increasing the specificity of the indirect ELISA.

@article{Conway1993ImmunodiagnosisOS,
  title={Immunodiagnosis of Strongyloides stercoralis infection: a method for increasing the specificity of the indirect ELISA.},
  author={David J Conway and Nina Atkins and Jared Lillywhite and J. W. Bailey and R. Daniel Robinson and John F. Lindo and Donald A. P. Bundy and Albert Edward Bianco},
  journal={Transactions of the Royal Society of Tropical Medicine and Hygiene},
  year={1993},
  volume={87 2},
  pages={
          173-6
        }
}
Indirect enzyme-linked immunosorbent assay (ELISA) allows sensitive detection of serum immunoglobulin (Ig) G against a soluble extract of Strongyloides stercoralis infective larvae. In this study, 40/40 (100%) human strongyloidiasis sera had high levels of anti-S. stercoralis IgG, but 30/40 (75%) filariasis sera, and 12/40 (30%) necatoriasis sera also had higher levels than control sera from UK residents. In attempts to increase the assay specificity by absorption of cross-reactive IgG, the… CONTINUE READING
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