Immunodetection of recombinant proteins based on antibodies directed against a metal binding peptide engineered for purification by immobilized metal affinity chromatography.

Abstract

Recently we reported (Evans, D.B., Tarpley W.G. and Sharma, S.K. (1991) Expression and characterization of chimeric rDNA proteins engineered for purification and cleavage. Protein Expr. Purif. 2, 205-213) a genetically engineered metal binding peptide (mbp) for the purification of recombinant proteins by immobilized metal affinity chromatography (IMAC… (More)

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@article{Evans1992ImmunodetectionOR, title={Immunodetection of recombinant proteins based on antibodies directed against a metal binding peptide engineered for purification by immobilized metal affinity chromatography.}, author={David B. Evans and Anne F Vosters and Joi Carter and Surendra K Sharma}, journal={Journal of immunological methods}, year={1992}, volume={156 2}, pages={231-8} }