Immunodetection of alpha1E voltage-gated Ca(2+) channel in chromogranin-positive muscle cells of rat heart, and in distal tubules of human kidney.

@article{Weiergrber2000ImmunodetectionOA,
  title={Immunodetection of alpha1E voltage-gated Ca(2+) channel in chromogranin-positive muscle cells of rat heart, and in distal tubules of human kidney.},
  author={Marco Weiergr{\"a}ber and Alexey Pereverzev and Rolf Vajna and M{\'e}laine Henry and Michael Schramm and Wolfgang Nastainczyk and Heike Grabsch and Toni Schneider},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={2000},
  volume={48 6},
  pages={807-19}
}
The calcium channel alpha1E subunit was originally cloned from mammalian brain. A new splice variant was recently identified in rat islets of Langerhans and in human kidney by the polymerase chain reaction. The same isoform of alpha1E was detected in rat and guinea pig heart by amplifying indicative cDNA fragments and by immunostaining using peptide-specific antibodies. The apparent molecular size of cardiac alpha1E was determined by SDS-PAGE and immunoblotting (218 +/- 6 kD; n = 3). Compared… CONTINUE READING

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