Immunodetection and partial cDNA sequence of the proteoglycan, superficial zone protein, synthesized by cells lining synovial joints.

@article{Schumacher1999ImmunodetectionAP,
  title={Immunodetection and partial cDNA sequence of the proteoglycan, superficial zone protein, synthesized by cells lining synovial joints.},
  author={Barbara L. Schumacher and Clare Hughes and Klaus E. Kuettner and Bruce Caterson and Margaret B. Aydelotte},
  journal={Journal of orthopaedic research : official publication of the Orthopaedic Research Society},
  year={1999},
  volume={17 1},
  pages={110-20}
}
We have previously described a large proteoglycan named superficial zone protein that was isolated and purified from culture medium of superficial slices of bovine articular cartilage. Monoclonal antibodies were raised against superficial zone protein and used as probes in Western blot analyses for immunohistochemical studies both to determine precisely which cells within the joint synthesize the proteoglycan and to isolate a cDNA fragment from a bovine chondrocyte lambdagt11 library that… CONTINUE READING