Immunocytochemistry of lysosomal hydrolases and their precursor forms in normal and mutant human cells

@article{Dongen1984ImmunocytochemistryOL,
  title={Immunocytochemistry of lysosomal hydrolases and their precursor forms in normal and mutant human cells},
  author={Jacques J. M. van Dongen and R. A. Barneveld and Hans J. Geuze and Hans Galjaard},
  journal={The Histochemical Journal},
  year={1984},
  volume={16},
  pages={941-954}
}
The acid hydrolases α-glucosidase, β-galactosidase,N-acetyl-β-d-hexosaminidase, β-glucocerebrosidase and cathepsin D were studied immunocytochemically in normal and mutant human cells using monoclonal and affinity-purified polyclonal antibodies. For light microscopy, Rhodamine or Fluorescein-labelled conjugates were used, and for electron microscopy protein A-gold conjugates were employed. With the double labelling procedure, it was found that in normal fibroblasts every lysosome contained all… CONTINUE READING

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Monoclonal antibodies against human flglucocerebrosidase

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