Immunocytochemical study of cell cycle control by cytokinin in cultured soybean cells

@article{Mader2004ImmunocytochemicalSO,
  title={Immunocytochemical study of cell cycle control by cytokinin in cultured soybean cells},
  author={Johanna C. Mader and David E. Hanke},
  journal={Journal of Plant Growth Regulation},
  year={2004},
  volume={15},
  pages={95-102}
}
An immunocytochemical method was used to determine the proportion of cells in the DNA synthesis (S phase) of the mitotic cycle in suspension cultures of soybean (Glycine max (L.) Merr. cv. Acme) callus of cotyledonary origin, the stably cytokinin-dependent tissue used in the cytokinin bioassay devised by Carlos O. Miller. A standard cell synchronization protocol involving hydroxyurea was used to demonstrate the applicability of the immunocytochemical method to this cell culture. Cells were… CONTINUE READING

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