Immunocytochemical localization of N-acetyl-aspartate with monoclonal antibodies

@article{Simmons1991ImmunocytochemicalLO,
  title={Immunocytochemical localization of N-acetyl-aspartate with monoclonal antibodies},
  author={Martha L. Simmons and Carmelita G. Frondoza and Joseph T. Coyle},
  journal={Neuroscience},
  year={1991},
  volume={45},
  pages={37-45}
}
N-Acetyl-aspartate is found in high concentrations in all areas of the brain, but is undetectable in non-neuronal tissue. In order to characterize the cellular localization of N-acetyl-aspartate in brain, highly specific monoclonal antibodies against N-acetyl-aspartate were produced by fusing spleen lymphocytes obtained from mice immunized with N-acetyl-aspartate conjugated to thyroglobulin by carbodiimide with P3/x63-Ag8.653 mouse myeloma cells. Clones were selected which secrete IgG2a(k… Expand
Differential distribution of N-acetylaspartylglutamate and N-acetylaspartate immunoreactivities in rat forebrain
TLDR
Although N-acetylaspartate-immunoreactivity was found in most neurons, it exhibited a great range of intensities between different neuronal types, and was observed in specific neuronal populations, including many groups thought to use GABA as a neurotransmitter. Expand
Localisation of N-acetylaspartate in oligodendrocytes/myelin
TLDR
Immunoelectron microscopy of the rat hippocampus and immunocytochemistry of carbodiimide-fixed adult rodent brain showed that N-acetylaspartate was concentrated in the myelin, supporting the notion that myelin damage is induced by the lack of N- acetyl aspartoacylase for lipid production. Expand
N-Acetylaspartate synthase is bimodally expressed in microsomes and mitochondria of brain.
TLDR
The tentative conclusion is that ANAT is bimodally targeted to MIT and a component of MIC-likely endoplasmic reticulum. Expand
N-Acetylaspartate in neuropsychiatric disorders
TLDR
Exogenous NAA is increasingly being exploited as a marker for functional and structural integrity of neurons in an expanding number of disorders, including Alzheimer's Disease and Huntington's Disease. Expand
N-Acetyl-Aspartate (NAA) Metabolism
N-Acetyl-Aspartate (NAA) remains a unique molecule in medicine, where although its been associated with a number of brain pathologies, primarily due to its non-invasive visibility by magneticExpand
Postmortem Degradation of N-Acetyl Aspartate and N-Acetyl Aspartylglutamate: An HPLC Analysis of Different Rat CNS Regions
TLDR
The data show a significant postmortem degradation of NAA and NAAG that needs to be considered when these compounds are studied ex-vivo. Expand
Evidence for mitochondrial and cytoplasmic N-acetylaspartate synthesis in SH-SY5Y neuroblastoma cells
TLDR
NAA biosynthesis occurs in both the cytoplasm and mitochondria of SH-SY5Y cells, and that the contribution from the mitochondrial compartment is quantitatively larger than that in the extramitochondrial compartment. Expand
N-Acetyl Aspartic Acid (NAA) and N-Acetyl Aspartylglutamic Acid (NAAG) in Human Ventricular, Subarachnoid, and Lumbar Cerebrospinal Fluid
TLDR
There did not appear to be any difference in lumbar fluid concentrations of either compound between control subjects, schizophrenic patients, Alzheimer's disease patients and a pooled group of patients with neurological degeneration, suggesting maintenance of their intracellular concentrations is probably energy dependent. Expand
N-acetylaspartylglutamate, N-acetylaspartate, and N-acetylated alpha-linked acidic dipeptidase in human brain and their alterations in Huntington and Alzheimer's diseases.
TLDR
It is shown that the decreases in the levels of NAAG and NAA and in the activity of NAALADase in AD and HD brain correlate primarily with neuronal loss, and that there is a close relationship between N AAG and the dipeptidase NAALadase in populations of affected neurons. Expand
N-acetylaspartate as an acetyl source in the nervous system.
TLDR
The results demonstrate that NAA metabolism is not restricted to the nervous system, although its biosynthesis is, and it is clear that acetyl moiety of NAA is incorporated into lipids and partially hydrolyzed to free acetate in both brain and liver preparations. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 26 REFERENCES
Production and characterization of monoclonal antibodies to N-acetyl-aspartyl-glutamate.
TLDR
Monoclonal antibodies significantly enhanced sensitivity of staining, allowing visualization of dorsal horn neurons in spinal cord, which were not readily detectable with polyclonal antiserum, and facilitates further clarification of the role of NAAG in the brain. Expand
Purification of specific antibody against aspartate and immunocytochemical localization of aspartergic neurons in the rat brain
TLDR
Results show that the antibody is a useful tool for the immunocytochemical demonstration of possible aspartergic neurons in the central nervous system, although the immunochemical expression of L-aspartate not used as a neurotransmitter must be taken into consideration. Expand
Quantitation of N-acetyl-aspartyl-glutamate in microdissected rat brain nuclei and peripheral tissues: findings with a novel liquid phase radioimmunoassay.
TLDR
The results support the previous conclusion that NAAG is co-localized in noradrenergic, serotonergic and cholinergic neurons. Expand
Selective immunocytochemical staining of mitral cells in rat olfactory bulb with affinity purified antibodies against N-acetyl-aspartyl-glutamate
TLDR
A specific neuronal localization of NAAG-like immunoreactivity is demonstrated and the candidacy of N AAG as a neurotransmitter of the lateral olfactory tract is supported. Expand
Specific antibodies against aspartate and their immunocytochemical application in the rat brain
TLDR
An immunological approach to visualize aspartate in the rat brain was attempted by raising antibodies against this acidic amino acid, and their titer and specificity were found high enough to allow their use in immunocytochemistry which demonstrated the presence of a large number of as partate-containing cell-bodies in many areas of the brain. Expand
Ontogenesis of N-acetyl-aspartate and N-acetyl-aspartyl-glutamate in rat brain.
TLDR
The results of this study suggest that NAAG is localized in a cell system that matures early and rises steadily from 15 DG to adulthood with a 30-fold increase in whole brain concentration. Expand
Hydrolysis of the brain dipeptide N-acetyl-L-aspartyl-L-glutamate. Identification and characterization of a novel N-acetylated alpha-linked acidic dipeptidase activity from rat brain.
TLDR
Comparison of the properties of NAALA dipeptidase to those of previously characterized enzymes suggests that this is a novel peptidase which may be involved in the synaptic degradation of N-acetyl-L-aspartyl- L-glutamate. Expand
STUDIES ON THE FUNCTION OF N‐ACETYL ASPARTIC ACID IN BRAIN
TLDR
It is concluded that in the mature animal NAA is metabolically inert and serves essentially as an anion with the apparent ability to shift reversibly from a dicarboxylic to a monocar boxylic acid form. Expand
Co‐localization of N‐acetyl‐aspartyl‐glutamate in central cholinergic, noradrenergic, and serotonergic neurons
An immunohistochemical technique for simultaneously visualizing two different antigens has been used to investigate the presence of the acidic dipeptide, N‐acetyl‐aspartyl‐glutamate (NAAG), inExpand
N‐Acetyl‐Aspartyl‐Glutamate: Regional Levels in Rat Brain and the Effects of Brain Lesions as Determined by a New HPLC Method
TLDR
Results suggest that NAAG may serve as an excitatory neurotransmitter in brain and the effects of selective brain lesions on their levels were examined, consistent with a neuronal localization of NAAg in brain. Expand
...
1
2
3
...