Immunocytochemical localization of actin in dendritic spines of the cerebral cortex using colloidal gold as a probe

@article{Cohen1985ImmunocytochemicalLO,
  title={Immunocytochemical localization of actin in dendritic spines of the cerebral cortex using colloidal gold as a probe},
  author={Rochelle S. Cohen and Sookja K Chung and Donald W. Pfaff},
  journal={Cellular and Molecular Neurobiology},
  year={1985},
  volume={5},
  pages={271-284}
}
1. Immunocytochemical localization of actin in rat cerebral cortex embedded in the resin LR White was performed using 5 nm colloidal gold as a probe. Antigenicity is maintained throughout the embedding procedure and the low electron opacity of LR White permits fine filamentous structures to be visualized. Control experiments included incubating the sections with normal goat serum or mouse IgG instead of the primary antibody, preadsorbing the antibody with actin from bovine muscle or liver… CONTINUE READING

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