Immunocytochemical evidence that GLUT4 resides in a specialized translocation post-endosomal VAMP2-positive compartment in rat adipose cells in the absence of insulin.

@article{Malide1997ImmunocytochemicalET,
  title={Immunocytochemical evidence that GLUT4 resides in a specialized translocation post-endosomal VAMP2-positive compartment in rat adipose cells in the absence of insulin.},
  author={Daniela A Malide and Nancy K. Dwyer and E. Joan Blanchette-Mackie and Samuel W. Cushman},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1997},
  volume={45 8},
  pages={1083-96}
}
Insulin stimulates glucose transport in rat adipose cells through the translocation of GLUT4 from a poorly defined intracellular compartment to the cell surface. We employed confocal microscopy to determine the in situ localization of GLUT4 relative to vesicle, Golgi, and endosomal proteins in these physiological insulin target cells. Three-dimensional analyses of GLUT4 immunostaining in basal cells revealed an intracellular punctate, patchy distribution both in the perinuclear region and… CONTINUE READING