Immunocytochemical evidence for the presence of oxytocin in rat testis

  title={Immunocytochemical evidence for the presence of oxytocin in rat testis},
  author={S. Guldenaar and B. Pickering},
  journal={Cell and Tissue Research},
SummaryThe presence of oxytocin, vasopressin and neurophysin in the testis of adult Wistar and Brattleboro rats has been examined immunocytochemically. After fixation in modified Bouin's solution, or Bouin's sublimate fixative, immunostaining was accomplished with the peroxidase-antiperoxidase method. The presence of immunoreactive oxytocin was demonstrated in 80% of the interstitial cell population of both rat strains while no staining was observed for vasopressin or neurophysin. 
Oxytocin in Leydig cells: an immunocytochemical study of Percoll-purified cells from rat testes
Measurement of basal testosterone production, histo-enzymological staining for 3β-hydroxysteroid dehydrogenase activity and electron microscopy indicated that the majority of Ley dig cells were found in fraction IV, indicating that the Leydig cells were also the oxytocin immunoreactive cells. Expand
Immunoreactive arginine vasopressin in the testis: immunocytochemical localization and testicular content in normal and in experimental cryptorchid mouse.
Staining for AVP and NPII was reduced in testes showing impaired spermatogenesis, and testicular immunoreactive (IR)-AVP content in normal pubertal and adult mice and in unilaterally cryptorchid animals was found. Expand
The effects of oxytocin and arginine vasopressin in vitro on epididymal contractility in the rat.
Findings suggest that both OT and AVP may have a role in regulating epididymal contractility and thus, perhaps, sperm transport through that organ. Expand
5α-Reductase Activity Increased by Oxytocin in the Rat Testis
Oxytocin is produced by the Leydig cells of the rat testis. In vivo chronic administration of the peptide into the testis results in reduced levels of testicular and plasma testosterone, but raisedExpand
Regulatory peptides in the mammalian urogenital system.
By immunocytochemistry a number of the gut/brain peptides have been demonstrated in nerve fibers of the mammalian urogenital tract, likely that impaired function of the peptidergic nerves is involved in sexual dysfunction such as male impotence. Expand
Central administration of mesotocin inhibits feeding behavior in chicks
Central injection of MT is associated with an anorexigenic response that does not appear CRH dependent in chicks, and neither plasma corticosterone concentration nor the mRNA expression of corticotrophin-releasing hormone (CRH) in the diencephalon was affected by ICV injection ofMT. Expand
Immunoreactivity to the pancreatic polypeptide family in the nervous system of the adult human blood fluke, Schistosoma mansoni
Results suggest that molecules with C-terminal homology to the PP-family are present in S. mansoni, which would appear to be important regulatory molecules in the parasite's nervous system and may play a role in the control of egg production. Expand
Expression of the prodynorphin gene in male and female mammalian reproductive tissues.
Investigation of male reproductive tissues of the rat, rabbit, and guinea pig as well as rat ovary and uterus all contain detectable levels of dynorphin immunoreactivity, suggesting that prodynorphin-derived peptides may exert paracrine and/or autocrine effects in mammalian reproductive tissues. Expand
Oxytocin promotes spermiation and sperm transfer in the mouse.
In the mouse oxytocin, whilst not involved in germ cell development, is important in the process of spermiation and sperm transfer in the mouse. Expand
Interactions between oxytocin- and calcium-modifying agents in the rat testicular capsule in vitro.
On the whole, it seems as if both intra- and extracellular calcium were involved in the contractile effect of oxytocin, although extacellular calcium does not seem to gain access to the cell through voltage-dependent calcium channels sensitive to selective calcium entry blockers. Expand


Immunocytochemical evidence for the presence of oxytocin and neurophysin in the large cells of the bovine corpus luteum
The simultaneous presence of immunoreactive oxytocin and immunore Active Oxytocin-neurophysin was demonstrated in large luteal cells of non-pregnant animals, while no staining for vasopressin or vasopressing peptides was observed. Expand
Immunohistochemical localization of neurophysin and oxytocin in the sheep corpora lutea
Antisera raised against neurophysin, oxytocin and vasopressin were used, in conjunction with the immunofluorescence procedure, to localize the neuropeptides in corpora luteal tissue collected fromExpand
Identification of oxytocin and vasopressin in the testis and in adrenal tissue
The concentrations of the hormone were too great to be explained by known circulating levels of the polypeptides, and their presence in steroid secreting organs suggests a possible role for them in steroidogenesis. Expand
Specificity of oxytocin and vasopressin immunofluorescence.
A total inhibition of immunofluorescence could not be obtained using the technique of preincubating either anti-vasopressin or anti-oxytocin plasmas with their homologous antigens. An alternativeExpand
Structure and function of rat testis through pubescence.
The histology, histochemistry, testosterone content and secretion of the rat testis were studied from 20 to 90 days of age and the disappearance of Leydig cell perinuclear basophilia was correlated with increasing Leydigs cell function. Expand
Is oxytocin an ovarian hormone?
The discovery of large amounts of oxytocin within the ovine corpus luteum reported here may provide an explanation of why there may exist different controls of reflex release and basal secretion of Oxytocin. Expand
Biosynthesis of oxytocin in the corpus luteum
Oxytocin biosynthesis Corpus luteum Bovine luteal cell Ovine luteal cell Oxytocin precursor
Long-term storage and regular repeated use of diluted antisera in glass staining jars for increased sensitivity, reproducibility, and convenience of single- and two-color light microscopic immunocytochemistry.
  • M. Sofroniew, U. Schrell
  • Chemistry, Medicine
  • The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
  • 1982
Diluted antisera, stored at 4 degrees C and continuously reused, were found to be stable for long periods of time, and consistently yielded high quality staining in both single- and two-color immunoperoxidase staining. Expand
More steroidogenic tissues containing oxytocin and vasopressin: human testis and fetal adrenal
  • Regul Pept
  • 1984
Immunocytochemistry: 2nd edn