Immunocytochemical distribution of extracellular matrix receptors in human osteoclasts: a beta 3 integrin is colocalized with vinculin and talin in the podosomes of osteoclastoma giant cells.

@article{ZamboninZallone1989ImmunocytochemicalDO,
  title={Immunocytochemical distribution of extracellular matrix receptors in human osteoclasts: a beta 3 integrin is colocalized with vinculin and talin in the podosomes of osteoclastoma giant cells.},
  author={A. Zambonin-Zallone and Anna Maria Teti and Maria Grano and Alessandro Rubinacci and M Abbadini and Mirella Gaboli and Pier Carlo Marchisio},
  journal={Experimental cell research},
  year={1989},
  volume={182 2},
  pages={645-52}
}
Human osteoclasts (OCLs) obtained from cell suspensions of surgically excised giant cell bone tumors (osteoclastomas) were attached to glass coverslips and analyzed by immunofluorescence with antibodies to integrins and cytoskeletal proteins. It was found that in OCLs (i) podosomes, identified by their F-actin core and by interference reflection microscopy, were predominantly found in a peripheral belt as described in avian OCLs; (ii) each F-actin core was surrounded by a ring of vinculin and… CONTINUE READING