Immunocytochemical detection of phosphatidylinositol 4,5-bisphosphate localization sites within the nucleus.

@article{Mazzotti1995ImmunocytochemicalDO,
  title={Immunocytochemical detection of phosphatidylinositol 4,5-bisphosphate localization sites within the nucleus.},
  author={Giovanni Mazzotti and Nicoletta Zini and Ermanno Rizzi and Ren{\'e} R Rizzoli and Angela Galanzi and Andrea Ognibene and Spartaco Santi and Alessandro Matteucci and Alberto Maria Martelli and N. M. Maraldi},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1995},
  volume={43 2},
  pages={181-91}
}
Phosphatidylinositol 4,5-bisphosphate (PIP2) is a key element of signal transduction, being the preferential substrate of specific phospholipases that produce second messengers such as inositol trisphosphate (IP3) and diacylglycerol (DG). Because PIP2 has been cytochemically identified by monoclonal antibodies not only in the cytoplasmic membranes but also in the nuclear envelope and within the nucleus, we performed a study by immunoblotting and by confocal and electron microscopic… CONTINUE READING