Immunocytochemical demonstration of visual pigments in the degenerate retinal and pineal photoreceptors of the blind cave salamander (Proteus anguinus)

  title={Immunocytochemical demonstration of visual pigments in the degenerate retinal and pineal photoreceptors of the blind cave salamander (Proteus anguinus)},
  author={Marjanca Kos and Boris Bulog and {\'A}goston Sz{\'e}l and P{\'a}l R{\"o}hlich},
  journal={Cell and Tissue Research},
Abstract. Visual pigments in the regressed eye and pineal of the depigmented neotenic urodele, the blind cave salamander (Proteus anguinus anguinus), were studied by immunocytochemistry with anti-opsin antibodies. The study included light- and electron-microscopic investigations of both the eye and the pineal organ. A comparison was made with the black pigmented subspecies Proteus anguinus parkelj (black proteus), which has a normal eye structure. In the retina of the black proteus, we found… 
Identification and characterization of visual pigments in caecilians (Amphibia: Gymnophiona), an order of limbless vertebrates with rudimentary eyes
Microspectrophotometry was used to determine the spectral sensitivities of the photoreceptors in three species of caecilian, Rhinatrema bivittatum, Geotrypetes seraphini and Typhlonectes natans, and rod opsin visual pigment, which may be associated with scotopic (dim light) vision when accompanied by other ‘rod-specific’ components of thePhototransduction cascade, was found to be present.
Evolutionary loss of cone photoreception in balaenid whales reveals circuit stability in the mammalian retina
These findings represent the first immunological and anatomical evidence of a naturally occurring rod‐monochromatic mammalian retina, and suggest that despite the loss of cone‐mediated photoreception, the associated cone signaling structures may be maintained for multichannel rod‐based signaling in balaenid whales.
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Using high-throughput RNA sequencing, the transcriptomes of different Acanthaster planci tissue samples are sequenced and analyzed, and the first evidence of an r-opsin photopigment expressed in a well developed visual eye in a deuterostome animal is found.
Pineal organs in deep demersal fish
Differences between day and night values and between autumn and spring suggest that pineal melatonin acts as neurochemical signal mediating rhythmic processes and behaviour.
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As Blind as a Bat? Opsin Phylogenetics Illuminates the Evolution of Color Vision in Bats
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Persistence of retinal dopamine cells in the degenerated eye of the cave salamander, Proteus anguinus L.
Results indicate a possible functional role of the remaining retina of the Proteus anguinus L. is a blind cave perennibranch amphibian whose visual system undergoes an important morphogenetic degeneration in adulthood.
Identification and distribution of photoreceptor subtypes in the neotenic tiger salamander retina.
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Binding sites of photoreceptor-specific antibodies COS-1, OS-2 and AO.
The chicken red-sensitive cone visual pigment (iodopsin) and several synthetic peptides of cone and rod visual pigments were used to find the binding sites of photoreceptor-specific antibodies with immunocytochemistry, providing direct evidence that both antibodies are specific to visual Pigments.
Photoreceptor cells in the Xenopus retina
This review summarizes the present state of knowledge about spectrally different photoreceptor cell types in the Xenopus retina based on morphology, combined with immunolabelling with various anti‐visual pigment antibodies and other molecular probes on semithin sections and retinal wholemounts.
The ciliary process probably plays its main role in the embryogenesis and regeneration of outer segments; and the dendrites and pigment epithelial processes in exchanges of material with the outer segments and perhaps with one another.
The ultrastructure of photoreceptor cells in the pineal organ of the blind cave salamander, Proteus anguinus (Amphibia, Urodela)
The ultrastructure of the photoreceptor cells in the pineal organ of blind, depigmented, neotenic cave salamander, Proteus anguinus, is studied to derive conclusions about their structure and function.
On the eyes and reactions to light of Proteus anguinus.
  • R. Hawes
  • Biology
    The Quarterly journal of microscopical science
  • 1945
It is argued that the condition of the eye and some other structures in the orbit is just as much the effect of neoteny as such typically embryonic features as the skin and gills and was present when Proteus first adopted a cavernicolous life.
Photoreceptor classes and transmission at the photoreceptor synapse in the retina of the clawed frog, Xenopus laevis
Mature rods of the Xenopus retina release glutamate in a calcium‐dependent manner and glutamate release was found to be a linear function of calcium influx through L‐type calcium channels.
Identification of cone classes in Xenopus retina by immunocytochemistry and staining with lectins and vital dyes.
It is suggested that the intermediate size, COS-1 negative cones are blue-sensitive based on the finding that blue- sensitive chromatic horizontal cells connect to them preferentially (Witkovsky et al., work in progress).
Immunocytochemical reactivity of Xenopus laevis retinal rods and cones with several monoclonal antibodies to visual pigments
Immunocytochemical reactions with several antibodies to visual pigments were used to study visual cells of the Xenopus laevis retina to detect bound antibodies with peroxidase at the light microscopic level.