Immunocytochemical characterization of neuron-rich rat brain primary cultures: calbindin D28K as marker of a neuronal subpopulation.

@article{Pfeiffer1989ImmunocytochemicalCO,
  title={Immunocytochemical characterization of neuron-rich rat brain primary cultures: calbindin D28K as marker of a neuronal subpopulation.},
  author={Barbara Pfeiffer and Anthony W. Norman and Bernd Hamprecht},
  journal={Brain research},
  year={1989},
  volume={476 1},
  pages={120-8}
}
The function in neurons of the vitamin D-dependent calcium ion-binding-protein of 28 kDa mol. wt., calbindin D28K, is unknown. In order to find a simple system for studying the function of this protein, neuron-rich primary cultures derived from brains of 16-day-old rat embryos were analyzed for the presence of calbindin D28K by immunocytochemical mono- and… CONTINUE READING