Immunocytochemical and biochemical analysis of carbonic anhydrase in primary astrocyte cultures from rat brain.

@article{Kimelberg1982ImmunocytochemicalAB,
  title={Immunocytochemical and biochemical analysis of carbonic anhydrase in primary astrocyte cultures from rat brain.},
  author={Harold K. Kimelberg and Philip Edwin Stieg and Joseph E. Mazurkiewicz},
  journal={Journal of neurochemistry},
  year={1982},
  volume={39 3},
  pages={734-42}
}
Carbonic anhydrase (CA) was studied in primary monolayer cultures from neonatal rat cerebral hemispheres with both immunocytochemical and biochemical techniques. In such cultures, which consist predominantly of astrocytes, immunocytochemical staining for CA using antibody raised against the type II enzyme from rat erythrocytes resulted in positive staining of the flat, glial fibrillary acidic protein-positive, astrocytic monolayer. Smaller, process-bearing, round cells that grew on top of the… CONTINUE READING