Immunochemistry, genuine size and tissue localization of collagen VI.

@article{Mark1984ImmunochemistryGS,
  title={Immunochemistry, genuine size and tissue localization of collagen VI.},
  author={Helga von der Mark and Monique Aumailley and Georg Wick and Raul Fleischmajer and Rupert Dr. Timpl},
  journal={European journal of biochemistry},
  year={1984},
  volume={142 3},
  pages={
          493-502
        }
}
Collagen VI was solubilized with pepsin from human placenta and used for preparing rabbit antisera. Major antigenic determinants were located in the central region of the antigen including triple-helical and globular structures. Antisera prepared against a constituent-chain showed preferential reactions with unfolded structures. Antibodies were purified by affinity chromatography and failed to cross-react with other collagen types I-V and with fibronectin. These antibodies demonstrated… CONTINUE READING

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