Immunochemical quantification of sarcoplasmic reticulum Ca2+-ATPase and calsequestrin in muscle biopsies from patients with myotonia congenita and paramyotonia congenita Eulenburg

Abstract

A sensitive enzyme-linked immunoadsorbant assay was developed to quantify Ca2+ -ATPase and calsequestrin from sarcoplasmic reticulum in human muscle biopsies. Tissue levels of Ca2+ -ATPase and calsequestrin averaged 51.5 ± 28.1 and 6.4 ± 1.8mg/g muscle protein, respectively, in control muscles (means ± SD, n=12). The high sensitivity and specificity of the… (More)
DOI: 10.1007/BF01283028

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