Immunochemical mapping of domains in human interleukin 4 recognized by neutralizing monoclonal antibodies.

@article{Ramanathan1993ImmunochemicalMO,
  title={Immunochemical mapping of domains in human interleukin 4 recognized by neutralizing monoclonal antibodies.},
  author={L. S. Ramanathan and Richard Ingram and Lee Sullivan and Robert G Greenberg and Richard Reim and Paul P. Trotta and Hung Viet Le},
  journal={Biochemistry},
  year={1993},
  volume={32 14},
  pages={3549-56}
}
Human interleukin 4 is a highly pleiotropic cytokine secreted by activated T cells that exerts multiple biological effects on B and T lymphocytes and other cell types. Elucidation of structure-function relations was accomplished by epitope mapping of a panel of monoclonal antibodies and by mutagenesis of selected amino acid residues. Epitope mapping of these monoclonal antibodies was achieved through binding studies with recombinant human interleukin 4 (rhuIL-4), proteolytic fragments produced… CONTINUE READING