Immunochemical isolation of vaccinia-virus antigens.
T Harry Birkbeck
John R. Stephen
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•Emergence of HIV-1 and AIDS; and success in stem cells and solid organ transplantation increased pool of patients with viral infections •Progress in antiviral treatment and availability of antiviral therapeutics •Development of advanced technologies (PCR, RT-PCR and ELISA, Multiplex analysis) •Increased risk of opportunistic viral infections in HIV-1 epidemics •Management of HIV-1 and hepatitis C infection required molecular diagnostics •Rapid test often determines the management of the case History •1898, Loeffler and Frosch found a filterable agent, they called virus, that caused foot an mouth disease of cattle •1892, Guarnieri found intranuclear and intracytoplasmic inclusion in small pox lesions •1929, complement fixation test by Bedson and Bland to detect antibodies to smallpox, vaccinia and varicella zoster virus •1948, Weller and Enders reported growth of viruses in tissue culture •1956, Liu and colleagues introduced fluorescent staining for the detection of influenza virus •1970s, development of monoclonal antibodies •1980s, development of ELISA and PCR assays for diagnostics
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