Immunochemical evidence for phytochrome regulation of the specific activity of ascorbate oxidase in mustard seedlings.

@article{Newbury1981ImmunochemicalEF,
  title={Immunochemical evidence for phytochrome regulation of the specific activity of ascorbate oxidase in mustard seedlings.},
  author={H. John Newbury and Harry Smith},
  journal={European journal of biochemistry},
  year={1981},
  volume={117 3},
  pages={575-80}
}
A monospecific antiserum to a Cucurbita ascorbate oxidase was shown to cross-react with the mustard enzyme. Purified antiserum was used in rocket immunoelectrophoresis plates to measure the amount of ascorbate oxidase protein in mustard cotyledon and hypocotyl extracts. On transfer from darkness to far-red light and activity of the enzyme in expanding cotyledons increased but the amount of enzyme protein remained the same. Unimbibed mustard seeds were shown to contain the same amount of… CONTINUE READING