Immunochemical detection of oxidatively damaged DNA

  title={Immunochemical detection of oxidatively damaged DNA},
  author={Pavel Rossner and Radim J. Sram},
  journal={Free Radical Research},
  pages={492 - 522}
Abstract Oxidatively damaged DNA is implicated in various diseases, including neurodegenerative disorders, cancer, diabetes, cardiovascular and inflammatory diseases as well as aging. Several methods have been developed to detect oxidatively damaged DNA. They include chromatographic techniques, the Comet assay, 32P-postlabelling and immunochemical methods that use antibodies to detect oxidized lesions. In this review, we discuss the detection of 8-oxo-7,8-dihydro-29-deoxyguanosine (8-oxodG… 

in double-stranded DNA using a photoelectrochemical DNA – BIOMARKER OF THE OXIDATIVE STRESS

The role of the 8-oxo-dG as a biomarker of oxidative stress and a predictor of diseases and results of the applied therapy is discussed.

Special issue on DNA oxidation: Mechanisms, measurement and consequences

  • M. Cooke
  • Biology
    Free radical research
  • 2012
Immunochemical approaches to the detection of oxidatively damaged DNA and 8-oxo-7,8dihydro-2¢ -deoxyguanosine (8-oxodG) in particular, are a popular alternative to chromatographic methods, not least due the availability of commercial kits for this purpose.

Biomarkers of nucleic acid oxidation – A summary state-of-the-art

Oxidative DNA damages by chemical exposures at work

This review concentrated on studies published between the years 2000 and 2012 that used to detect 8-oxodG in humans, laboratory animals and in cell lines, suggesting that biomarkers may be responsible for understanding the role of oxidative DNA damage in disease.

Oxidative stress and inflammation generated DNA damage by exposure to air pollution particles.

Effects of ionizing radiation on biological molecules--mechanisms of damage and emerging methods of detection.

Chemical mechanisms for IR-induced modifications of biomolecules along with methods for their detection are described and the synergy of combined "omics" technologies such as genomics and epigenomics, proteomics, and metabolomics is highlighted.

Molecular Epidemiology Focused on Airborne Carcinogens

Health risk associated with genotoxic and carcinogenic effects of air pollution is evaluated by biomarkers of exposure, effect and susceptibility. The use of these molecular methods combined with

Free radical and superoxide reactivity detection in semen quality assessment: past, present, and future

The aim of this review is to provide information on the currently available methods to assess and quantify ROS and particularly superoxide in male reproductive cells, tissues, and fluids which may have a significant clinical utility in identifying men with impaired fertility associated with oxidative stress.

Analysis of biomarkers in a Czech population exposed to heavy air pollution. Part II: chromosomal aberrations and oxidative stress.

A positive relationship was observed between lipid peroxidation and air pollution; protein oxidation seems to be positively affected by PM2.5 in both regions.



DNA Damage, a Biomarker of Carcinogenesis: Its Measurement and Modulation by Diet and Environment

  • E. HwangP. Bowen
  • Biology, Chemistry
    Critical reviews in food science and nutrition
  • 2007
The objective of this review is to discuss the biological significance of oxidative DNA damage, evaluate the effectiveness of several techniques for measurement of oxidativeDNA damage in various biological samples and review current research on factors that influence DNA oxidative damage using these techniques.

Increased oxidative DNA damage, 8-hydroxydeoxy- guanosine, in human pterygium

The increased levels of 8-OHdG in the pteryGium tissues indicate that oxidative stress could play a role in the development of pterygium and are useful in the prevention and treatment of this disease.

Ameliorating effect of N-acetylcysteine and curcumin on pesticide-induced oxidative DNA damage in human peripheral blood mononuclear cells

Results indicate that pesticide-induced oxidative stress is probably responsible for the DNA damage, and N-acetylcysteine or curcumin attenuate this effect by counteracting the oxidative stress.

Measurement and Meaning of Oxidatively Modified DNA Lesions in Urine

The formation of the European Standards Committee on Urinary (DNA) Lesion Analysis is reported, whose primary goal is to achieve consensus between methods and establish reference ranges in health and disease.

Isocyanates induces DNA damage, apoptosis, oxidative stress, and inflammation in cultured human lymphocytes

A dose‐ and time‐dependent response of methyl isocyanate (MIC) on cultured human lymphocytes isolated from healthy human volunteers is shown, providing evidence to hitherto unknown molecular mechanisms of immunotoxic consequences of isOCyanate exposure at a genomic level.

Oxidative DNA Damage and Antioxidant Activity in Patients with Inflammatory Bowel Disease

Plasma levels of 8-OHdG, SOD, and G-Px activity were found to be increased in the patient group compared to the control group and this may explain the increased risk of developing colon cancer in these patients.

8-hydroxy-2′ -deoxyguanosine (8-OHdG): A Critical Biomarker of Oxidative Stress and Carcinogenesis

The biomarker 8-OHdG or 8-oxodG has been a pivotal marker for measuring the effect of endogenous oxidative damage to DNA and as a factor of initiation and promotion of carcinogenesis and has been used to estimate the DNA damage in humans after exposure to cancer-causing agents.

High DNA Oxidative Damage in Systemic Sclerosis

Increased values of 8-oxodG in SSc patients and a relevant association with a fibrotic phenotype are found and the predictive value of this marker and its potential influence on Fibrotic disturbances remain to be determined.