Immunochemical and biosynthetic analysis of monoclonal antibody-defined melanoma-associated antigen.

@article{Bumol1982ImmunochemicalAB,
  title={Immunochemical and biosynthetic analysis of monoclonal antibody-defined melanoma-associated antigen.},
  author={Thomas F. Bumol and Darwin O. Chee and Ralph A. Reisfeld},
  journal={Hybridoma},
  year={1982},
  volume={1 3},
  pages={283-92}
}
Immunoprecipitation studies with application of monoclonal antibody F11 originally made to a partially purified spent medium antigen of melanoma cells, made it possible to delineate the molecular profiles of both the cell associated and spent medium antigens recognized on melanoma cells intrinsically labeled with glycoprotein precursors. F11 distinguishes a glycoprotein of Mr 100,000 (100 K) in the spent media of melanoma cells while a parallel analysis of detergent lysates of cells reveals a… CONTINUE READING