Immunochemical and biochemical analysis of the proliferating cell nuclear antigen (PCNA) in HeLa cells.

@article{Sadaie1986ImmunochemicalAB,
  title={Immunochemical and biochemical analysis of the proliferating cell nuclear antigen (PCNA) in HeLa cells.},
  author={Mohammad Reza Sadaie and Michael B. Mathews},
  journal={Experimental cell research},
  year={1986},
  volume={163 2},
  pages={423-33}
}
PCNA, also known a cyclin, a protein of molecular weight (MW) 35 000, accumulates in the nuclei of dividing and transformed cells and reacts with autoantibodies from certain lupus patients. Using an indirect immunofluorescence technique, we show that lupus sera containing anti-PCNA antibodies reveal a heterogeneous nuclear fluorescence pattern upon reaction… CONTINUE READING