Immunochemical Properties of Recombinant Ompf Porin from Outer Membrane of Yersinia pseudotuberculosis

@inproceedings{Portnyagina2012ImmunochemicalPO,
  title={Immunochemical Properties of Recombinant Ompf Porin from Outer Membrane of Yersinia pseudotuberculosis},
  author={Olga Yu Portnyagina and Olga V. Sidorova and Valentina A Khomenko and Olga D. Novikova and Marina P Issaeva and Tamara F. Solov’eva},
  year={2012}
}
Porins from outer membranes (OM) of Gram-negative bacteria exist in intact membranes as homotrimers forming water-filled pores or channels that provide low-molecular compounds transportation. Surface localization of the porins and their structural peculiarities determine multiplicity of their functions except for the transport one. On the one hand, porins are targets for the host immune system. As the targets they activate factors of the organism fast protection and are involved in the… Expand
Production of recombinant porin from Y. pseudotuberculosis in a water-soluble form for pseudotuberculosis diagnostics
TLDR
OmpF porin from the outer membrane of Yersinia pseudotuberculosis was cloned into pET-40b(+) plasmid and was shown to have the ordered spatial structure at the levels of secondary and tertiary structure. Expand
Molecular Characterization, Phylogenetic, Expression, and Protective Immunity Analysis of OmpF, a Promising Candidate Immunogen Against Yersinia ruckeri Infection in Channel Catfish
TLDR
Re recombinant OmpF as a subunit vaccine was injected with commercial adjuvant ISA763, significantly enhanced the immune response by increasing serum antibody levels, lysozyme activity, complement C3 activity, total protein content, SOD activity, immune-related genes expression in the head kidney and spleen, and survival percent of channel catfish against Y. ruckeri infection. Expand

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TLDR
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