Immunochemical Assay for Recognition of 2S-Glutathionyl Acetate , a Glutathione Conjugate Derived from 1 , 1-Dichloroethylene-Epoxide 1

@inproceedings{Forkert1997ImmunochemicalAF,
  title={Immunochemical Assay for Recognition of 2S-Glutathionyl Acetate , a Glutathione Conjugate Derived from 1 , 1-Dichloroethylene-Epoxide 1},
  author={P. G. Forkert and Kathy S Collins and T F Dowsley and Gregory M Ross},
  year={1997}
}
Cytotoxicities induced by 1,1-dichloroethylene (DCE) are ascribed to cytochrome P450-dependent metabolism to an epoxide. Conjugation of the DCE-epoxide with glutathione (GSH) results in the formation of the conjugates 2-S-glutathionyl acetate (GTA) and 2-(S-glutathionyl) acetyl glutathione (GAG); GAG undergoes hydrolysis to form GTA, and thus GTA is a major metabolite of DCE metabolism. Our objective is to develop an antiserum against the chemically synthesized GTA, and for immunization, we… CONTINUE READING