Immunoblotting analysis of sera from patients with candidal vaginitis and healthy females.

@article{Ishiguro1992ImmunoblottingAO,
  title={Immunoblotting analysis of sera from patients with candidal vaginitis and healthy females.},
  author={Akira Ishiguro and Morio Homma and Tetsuo Sukai and Koji Higashide and Shinpei Torii and Kiyoshi Tanaka},
  journal={Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology},
  year={1992},
  volume={30 4},
  pages={
          281-92
        }
}
Antigenic components of Candida albicans were extracted from whole cells with a buffer containing SDS and 2-mercaptoethanol, and separated by SDS-polyacrylamide gel electrophoresis. The components reactive with IgG, IgA, IgM and IgE antibodies in sera from patients with (14 subjects) and without (15 subjects) C. albicans in the vagina, and from healthy females (34 subjects), were investigated by immunoblotting using immunoglobulin class-specific antibodies. Many components reacted with IgG and… CONTINUE READING