Immunoblot Analyses of the Relative Contributions of Cysteine and Aspartic Proteases to Neurofilament Breakdown Products Following Experimental Brain Injury in Rats

@article{Posmantur1998ImmunoblotAO,
  title={Immunoblot Analyses of the Relative Contributions of Cysteine and Aspartic Proteases to Neurofilament Breakdown Products Following Experimental Brain Injury in Rats},
  author={R. M. Posmantur and Xia Zhao and Andreas Kampfl and Guy L. Clifton and Ronald L. Hayes},
  journal={Neurochemical Research},
  year={1998},
  volume={23},
  pages={1265-1276}
}
Analyses using either one or two-dimensional gel electrophoresis were performed to identify the contribution of several proteases to lower molecular weight (MW) neurofilament 68 (NF68) break down products (BDPs) detected in cortical homogenates following unilateral cortical impact injury in rats. One dimensional immunoblot of BDPs obtained from in vitro cleavage of enriched neurofilaments (NF) by purified μ-calpain, m-calpain, cathepsin, B, cathepsin D, and CPP32 (caspase-3) were compared to in… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 11 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 88 references

Degradation of neu- rofilaments by neutral calcium activated proteases. In: CRC's In- tracellular calcium dependent proteolysis

  • W. Schlaepfer, U. P. Zimmerman
  • (Mellegren RL,
  • 1990
Highly Influential
4 Excerpts

A quantitative measure of calpain activity following traumatic brain injury using casein zymography

  • X. Zhao, R. Posmantur, J. S. Liu, A. Kampfl, G. L. Clifton, R. L. Hayes
  • Journal of Cerebral Blood Flow Metab
  • 1997
3 Excerpts

Examination of of calpain 1 specific break-down products to A-spectrin in a controlled cor- tical impact model at both early and late timepoints

  • J. K. Newcomb, S. J. Liu, +4 authors R. L. Hayes
  • J. Neuro- trauma 6:369-383
  • 1997
2 Excerpts

Similar Papers

Loading similar papers…