Immunobiosensor chips for detection of Escherichia coil O157:H7 using electrochemical impedance spectroscopy.

@article{Ruan2002ImmunobiosensorCF,
  title={Immunobiosensor chips for detection of Escherichia coil O157:H7 using electrochemical impedance spectroscopy.},
  author={Chuanmin Ruan and Liju Yang and Yanbin Li},
  journal={Analytical chemistry},
  year={2002},
  volume={74 18},
  pages={4814-20}
}
Impedance biosensor chips were developed for detection of Escherichia coli O157:H7 based on the surface immobilization of affinity-purified antibodies onto indium tin oxide (ITO) electrode chips. The immobilization of antibodies onto ITO chips was carried out using an epoxysilane monolayer to serve as a template for chemical anchoring of antibodies. The surface characteristics of chips before and after the binding reaction between the antibodies and antigens were characterized by atomic force… CONTINUE READING