Immunobead RT-PCR: a sensitive method for detection of circulating tumor cells.

@article{Eaton1997ImmunobeadRA,
  title={Immunobead RT-PCR: a sensitive method for detection of circulating tumor cells.},
  author={Michael Eaton and Jennifer E Hardingham and Dusan Kotasek and Alexander Dobrovic},
  journal={BioTechniques},
  year={1997},
  volume={22 1},
  pages={100-5}
}
Detection of circulating tumor cells and micrometastases in patients with cancer should prove useful in determining prognosis and in planning and monitoring systemic therapies. We have developed immunomagnetic isolation of carcinoma cells followed by reverse transcription polymerase chain reaction (immunobead RT-PCR) as a method for identifying very small numbers of breast cancer cells in blood. The expression of cytokeratin 19 (K19) was used as the marker by which the isolated tumor cells were… CONTINUE READING