Immunoassay using antigen—enzyme conjugates

@article{vanWeemen1971ImmunoassayUA,
  title={Immunoassay using antigen—enzyme conjugates},
  author={B.K. van Weemen and Alana Schuurs},
  journal={FEBS Letters},
  year={1971},
  volume={15}
}
Alternative Approaches to Enzyme Immunoassays
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Two lines of development have now emerged in which enzyme immunoassays seem to offer preferable alternatives to currently used methods, including radioimmunoassay (RIAs), and the first one is in the measurement of haptens for which homogeneous enzyme immunOassay techniques can be developed.
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The Enzyme Linked Immunosorbent Assay (ELISA)
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Radioimmunoassay, enzyme and non-enzyme-based immunoassays.
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The majority of RIA assay formats recommend sample cleaning and concentration (particularly when analyte concentration and assay sensitivity is low), although a large number of ELISA assays can cope with direct use of unprocessed plasma.
Enzyme-amplified immunoassays.
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