Immunoassay of three enolase isozymes in human serum and in blood cells.

  title={Immunoassay of three enolase isozymes in human serum and in blood cells.},
  author={Koichi Kato and Ryoichi Asai and Akio Shimizu and Fumiko Suzuki and Yutaka Ariyoshi},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  volume={127 3},
Sandwich enzyme immunoassay procedures for measurement of three human enolase isozymes (alpha alpha, alpha gamma and gamma gamma forms) were developed by use of purified antibodies specific to the alpha or the gamma subunit of enolase. The assay systems consisted of polystyrene balls with immobilized antibody F(ab')2 fragments and antibody Fab' fragments labeled with beta-D-galactosidase from E. coli. The measurable range was from 3 pg to 10 ng of each enolase isozyme per assay tube, and the… CONTINUE READING
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