Immunoassay method for quantitative determination of nisin in solution and on polymeric films.

@article{Leung2002ImmunoassayMF,
  title={Immunoassay method for quantitative determination of nisin in solution and on polymeric films.},
  author={Patricia P N Leung and Mohammed A Khadre and Thomas H Shellhammer and Ahmed Yousef},
  journal={Letters in applied microbiology},
  year={2002},
  volume={34 3},
  pages={199-204}
}
AIMS To develop a non-competitive direct immunoassay method for quantifying nisin in solution or adsorbed to surfaces. METHODS AND RESULTS The developed method differs from traditional ELISA in pre-forming a complex of the nisin antibodies and the secondary antibody conjugate before addition to the nisin-coated solid phase. The modifications may help decrease interference from the nisin degradation molecules and reduce non-specific adsorption of secondary antibody conjugate. The new method… CONTINUE READING

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