Immunoaffinity profiling of tyrosine phosphorylation in cancer cells

  title={Immunoaffinity profiling of tyrosine phosphorylation in cancer cells},
  author={J. Rush and A. Moritz and K. Lee and A. Guo and V. L. Goss and E. J. Spek and Hui Bin Zhang and X. Zha and R. Polakiewicz and M. Comb},
  journal={Nature Biotechnology},
  • J. Rush, A. Moritz, +7 authors M. Comb
  • Published 2005
  • Biology, Medicine
  • Nature Biotechnology
  • Tyrosine kinases play a prominent role in human cancer, yet the oncogenic signaling pathways driving cell proliferation and survival have been difficult to identify, in part because of the complexity of the pathways and in part because of low cellular levels of tyrosine phosphorylation. In general, global phosphoproteomic approaches reveal small numbers of peptides containing phosphotyrosine. We have developed a strategy that emphasizes the phosphotyrosine component of the phosphoproteome and… CONTINUE READING
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