Immunoaffinity profiling of tyrosine phosphorylation in cancer cells

@article{Rush2005ImmunoaffinityPO,
  title={Immunoaffinity profiling of tyrosine phosphorylation in cancer cells},
  author={John Rush and Albrecht Moritz and Kimberly A. Lee and Ailan Guo and Valerie L Goss and Erik J. Spek and Hui Zhang and Xiangming Zha and Roberto D. Polakiewicz and Michael Comb},
  journal={Nature Biotechnology},
  year={2005},
  volume={23},
  pages={94-101}
}
Tyrosine kinases play a prominent role in human cancer, yet the oncogenic signaling pathways driving cell proliferation and survival have been difficult to identify, in part because of the complexity of the pathways and in part because of low cellular levels of tyrosine phosphorylation. In general, global phosphoproteomic approaches reveal small numbers of peptides containing phosphotyrosine. We have developed a strategy that emphasizes the phosphotyrosine component of the phosphoproteome and… Expand
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