Immunoaffinity chromatography and a biotin-streptavidin amplified enzymeimmunoassay for sensitive and specific estimation of estradiol-17 beta.

@article{Meyer1990ImmunoaffinityCA,
  title={Immunoaffinity chromatography and a biotin-streptavidin amplified enzymeimmunoassay for sensitive and specific estimation of estradiol-17 beta.},
  author={Heinrich H. D. Meyer and Helga Sauerwein and Benezeth M. Mutayoba},
  journal={Journal of steroid biochemistry},
  year={1990},
  volume={35 2},
  pages={263-9}
}
A sensitive test system has been developed for estimation of estradiol-17 beta (E2) in bovine plasma. Plasma extracts are first purified by a selective immunoaffinity chromatography (IAC) using an antibody raised against estradiol-6-carboxymethyloxime-bovine serum albumin and immobilized to Sepharose. The eluate was analysed by a competitive enzyme… CONTINUE READING