Immunoaffinity chromatographic analysis for purification of specific diagnostic antigens of Paramphistomum epiclitum.

@article{Arora2010ImmunoaffinityCA,
  title={Immunoaffinity chromatographic analysis for purification of specific diagnostic antigens of Paramphistomum epiclitum.},
  author={Ritu Arora and Nirbhay K. Singh and Prayag Dutt Juyal and Jyoti and Srikanta Ghosh},
  journal={Journal of parasitic diseases : official organ of the Indian Society for Parasitology},
  year={2010},
  volume={34 1},
  pages={57-61}
}
Polypeptide profile of somatic antigen of Paramphistomum epiclitum (PSAg) and Gastrothylax crumenifer (GSAg) was studied by SDS-PAGE. PSAg and GSAg showed 14 and 19 polypeptides in the range of 14.9-95.5 and 13.7-129.6 kDa with six common polypeptides of mol wt 16.8, 21.8, 23.7, 35.5, 43.4 and 70.8 kDa. P. epiclitum experimentally infected sheep sera were used for identification of specific immuno-dominant peptide in the range of 37-40 kDa against P. epiclitum by western blotting. Hyperimmune… CONTINUE READING