Immuno-spin trapping analyses of DNA radicals

  title={Immuno-spin trapping analyses of DNA radicals},
  author={D C Ramirez and Sandra E. Gomez-Mejiba and Ronald Paul Mason},
  journal={Nature Protocols},
Immuno-spin trapping is a highly sensitive method for detecting DNA radicals in biological systems. This technique involves three main steps: (i) in situ and real-time trapping of DNA radicals with the nitrone spin trap 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), thus forming DMPO–DNA nitrone adducts (referred to here as nitrone adducts); (ii) purification of nitrone adducts; and (iii) analysis of nitrone adducts by heterogeneous immunoassays using Abs against DMPO. In experiments, DMPO is added… CONTINUE READING