Immuno-slot-blot: A highly sensitive immunoassay for the quantitation of carcinogen-modified nucleosides in DNA

@article{Nehls1984ImmunoslotblotAH,
  title={Immuno-slot-blot: A highly sensitive immunoassay for the quantitation of carcinogen-modified nucleosides in DNA},
  author={Peter Nehls and J{\"u}rgen Adamkiewicz and Manfred F. Rajewsky},
  journal={Journal of Cancer Research and Clinical Oncology},
  year={1984},
  volume={108},
  pages={23-29}
}
We have established a highly sensitive immuno-slot-blot (ISB) procedure that can be routinely applied for detection and quantitation of any heat- or alkali-stable structural DNA modification (caused by carcinogens or mutagens, for example) for which a specific (monoclonal) antibody (MAB) is available. The essential step in this assay is the immobilization… CONTINUE READING