Immuno-enzymatic assay for the detection of Schistosoma mansoni antigens in serum of mice harbouring bisexual or unisexual light worm infections.

@article{Ferreira1979ImmunoenzymaticAF,
  title={Immuno-enzymatic assay for the detection of Schistosoma mansoni antigens in serum of mice harbouring bisexual or unisexual light worm infections.},
  author={Ant{\^o}nio Walter Ferreira and Anna Lucia Caldini and Sumie Hoshino-Shimizu and M{\'a}rio Endsfeldz Camargo},
  journal={Journal of helminthology},
  year={1979},
  volume={53 3},
  pages={
          189-94
        }
}
Enzyme-linked immunosorbent assay (ELISA) double antibody technique was standardized for detecting S. mansoni polysaccharide and protein antigens in serum of infected mice. Anti-sera specific for either worm components were obtained in sheep and peroxidase conjugates prepared from each serum. The immunoenzimatic test for protein could detect as little as 6 micrograms/ml antigens, and the test for polysaccharides 3 micrograms/ml. Both bisexual and unisexual male worm low infections were produced… CONTINUE READING
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