Immuno-Isolating Dual Poly(ethylene glycol) Capsule Prevents Cancer Cells from Spreading Following Mouse Ovarian Tissue Auto-Transplantation

  title={Immuno-Isolating Dual Poly(ethylene glycol) Capsule Prevents Cancer Cells from Spreading Following Mouse Ovarian Tissue Auto-Transplantation},
  author={J. Day and A. David and C. Long and Grace G Bushnell and T. Woodruff and L. Shea and A. Shikanov},
  journal={Regenerative medicine frontiers},
For female cancer survivors, premature ovarian insufficiency (POI) is a common complication of anticancer treatments. Ovarian tissue cryopreservation before treatment, followed by auto-transplantation after remission is a promising option to restore fertility and ovarian endocrine function. However, auto-transplantation is associated with the risk of re-introducing malignant cells harbored in the stroma of the ovarian autograft. To mitigate this risk, we investigated in this pilot study whether… Expand
1 Citations
Encapsulated allografts preclude host sensitization and promote ovarian endocrine function in ovariectomized young rhesus monkeys and sensitized mice
It is demonstrated, for the first time, that encapsulation of ovarian allografteds prevents sensitization and protects the allograft from rejection in young rhesus monkeys and in sensitized mice. Expand


Immunoisolating poly(ethylene glycol) based capsules support ovarian tissue survival to restore endocrine function.
It is demonstrated for the first time that immunoisolating PEG-VS capsules can support ovarian follicular development resulting in the restoration of ovarian endocrine function and can be applied to future allogeneic studies. Expand
Restoring Ovarian Endocrine Function with Encapsulated Ovarian Allograft in Immune Competent Mice
Results show that TheraCyte effectively isolates the graft from immune recognition but also supports follicular growth, which was confirmed by decreased serum FSH levels in ovariectomized mice after implantation. Expand
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The ability to obtain live births by transplanting isolated primordial and primary follicles, while also reducing the risk of re-seeding disease relative to ovarian tissue transplantation, may ultimately provide a means to preserve fertility in premenopausal oncology patients. Expand
Is transplantation of a few leukemic cells inside an artificial ovary able to induce leukemia in an experimental model?
Transplantation of 100 leukemic cells appears to be insufficient to induce leukemia after 20 weeks, and results in an immunodeficient xenografting model are quite reassuring, however, for clinical application, follicle suspensions must be purged of leukedmic cells before grafting, as even the slightest risk should be avoided. Expand
Is transplantation of cryopreserved ovarian tissue from patients with advanced-stage breast cancer safe? A pilot study
This pilot study is the first to evaluate the risk of contamination of cryopreserved ovarian tissue from advanced-stage breast cancer patients by xenotransplantation for 6 months to immunodeficient mice, associated with more conventional screening methods. Expand
Retrievable hydrogels for ovarian follicle transplantation and oocyte collection
Findings demonstrate a viable option for safe use of potentially cancer‐laden ovarian donor tissue for in vivo follicle maturation within a retrievable hydrogel and subsequent oocyte collection, and may provide novel options to preserve fertility for young female patients with cancer. Expand
Initiation of puberty in mice following decellularized ovary transplant.
It is tested whether a decellularized ovarian scaffold could be created, recellularized and transplanted to initiate puberty in mice, providing data that could be used to drive future human transplants and have broader implications on the bioengineering of other organs with endocrine function. Expand
Reimplantation of cryopreserved ovarian tissue from patients with acute lymphoblastic leukemia is potentially unsafe.
By quantitative RT-PCR, ovarian contamination by malignant cells in acute as well as chronic leukemia is demonstrated, whereas histology fails to do so, and chemotherapy before ovarian cryopreservation does not exclude malignant contamination. Expand
Risk of transferring malignant cells with transplanted frozen-thawed ovarian tissue.
For ovarian tissue from patients with hematologic malignancies, it is of paramount importance to identify minimal residual disease before ovarian tissue transplantation, and these pathologies are considered to be most at risk of ovarian metastasis. Expand
Bioengineering the ovarian follicle microenvironment.
This review describes the application of tissue engineering principles to promote ovarian follicle maturation and produce mature oocytes through either in vitro culture or transplantation. Expand