Immuno-Golgi as a Tool for Analyzing Neuronal 3D-Dendritic Structure in Phenotypically Characterized Neurons

@inproceedings{Pinto2012ImmunoGolgiAA,
  title={Immuno-Golgi as a Tool for Analyzing Neuronal 3D-Dendritic Structure in Phenotypically Characterized Neurons},
  author={Luisa R. Pinto and Antonio Mateus-Pinheiro and M{\'o}nica Morais and Jo{\~a}o Miguel Bessa and Nuno Sousa},
  booktitle={PloS one},
  year={2012}
}
Characterization of neuronal dendritic structure in combination with the determination of specific neuronal phenotype or temporal generation is a challenging task. Here we present a novel method that combines bromodioxyuridine (BrdU) immunohistochemistry with Golgi-impregnation technique; with this simple non-invasive method, we are able to determine the tridimensional structure of dendritic arborization and spine shape of neurons born at a specific time in the hippocampus of adult animals… CONTINUE READING

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