Immuno‐Quantitative Polymerase Chain Reaction for Detection and Quantitation of Prion Protein

  title={Immuno‐Quantitative Polymerase Chain Reaction for Detection and Quantitation of Prion Protein},
  author={S. Gofflot and B. El Moualij and D. Zorzi and L. Melen and S. Roels and Dominique Quatpers and J. Grassi and E. Vanopdenbosch and E. Heinen and W. Zorzi},
  journal={Journal of Immunoassay and Immunochemistry},
  pages={241 - 258}
Abstract Immuno‐polymerase chain reaction (PCR) is an extremely sensitive detection method, combining the specificity of antibody detection and the sensitivity of PCR. We have developed an immuno‐quantitative PCR (iqPCR), exploiting real‐time PCR technology, in order to improve this immuno‐detection method and make it quantitative. To illustrate the advantages of iqPCR, we have compared it with a conventional enzyme linked immuno sorbent assay (ELISA) technique in experiments aimed at detecting… Expand
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  • Wu, Huang, Lai, Shaio
  • Chemistry, Medicine
  • Letters in applied microbiology
  • 2001
Sensitive detection of pathological prion protein by cyclic amplification of protein misfolding