Immuno‐Quantitative Polymerase Chain Reaction for Detection and Quantitation of Prion Protein

@article{Gofflot2004ImmunoQuantitativePC,
  title={Immuno‐Quantitative Polymerase Chain Reaction for Detection and Quantitation of Prion Protein},
  author={St{\'e}phanie Gofflot and Benaissa El Moualij and Dani{\`e}le Zorzi and Laurence Melen and Stefan Roels and Dominique Quatpers and Jacques Grassi and Emmanuel Vanopdenbosch and Ernst Heinen and Willy Zorzi},
  journal={Journal of Immunoassay and Immunochemistry},
  year={2004},
  volume={25},
  pages={241 - 258}
}
Abstract Immuno‐polymerase chain reaction (PCR) is an extremely sensitive detection method, combining the specificity of antibody detection and the sensitivity of PCR. We have developed an immuno‐quantitative PCR (iqPCR), exploiting real‐time PCR technology, in order to improve this immuno‐detection method and make it quantitative. To illustrate the advantages of iqPCR, we have compared it with a conventional enzyme linked immuno sorbent assay (ELISA) technique in experiments aimed at detecting… Expand
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