Immunization of mice with a DNA vaccine based on severe acute respiratory syndrome coronavirus spike protein fragment 1.

@article{Zhao2006ImmunizationOM,
  title={Immunization of mice with a DNA vaccine based on severe acute respiratory syndrome coronavirus spike protein fragment 1.},
  author={Bo Zhao and Ningyi Jin and Rui-lin Wang and Li-shu Zhang and Ying-jiu Zhang},
  journal={Viral immunology},
  year={2006},
  volume={19 3},
  pages={
          518-24
        }
}
According to data in GenBank, a gene encoding SARS spike protein fragment 1 (S1) was synthesized. After recombination with an immunostimulatory sequence (ISS), the gene was cloned into the plasmid pIRES to produce pIRES-ISS-S1. On confirmation of the expression of S1 protein by indirect immunofluorescence assay (IFA), after the transfection of pIRES-ISS-S1 into BHK-21 cells, the DNA vaccine was repeatedly administrated to BALB/c mice. CD4+ and CD8+ spleen T lymphocytes were analyzed by flow… CONTINUE READING