Immune profiling with a Salmonella Typhi antigen microarray identifies new diagnostic biomarkers of human typhoid

@article{Liang2013ImmunePW,
  title={Immune profiling with a Salmonella Typhi antigen microarray identifies new diagnostic biomarkers of human typhoid},
  author={Li Liang and Silvia Ju{\'a}rez and Tran Vu Thieu Nga and Sarah J. Dunstan and Rie Nakajima-Sasaki and D. Huw Davies and Stephen J. McSorley and Stephen Baker and Philip L. Felgner},
  journal={Scientific Reports},
  year={2013},
  volume={3}
}
Current serological diagnostic assays for typhoid fever are based on detecting antibodies against Salmonella LPS or flagellum, resulting in a high false-positive rate. Here we used a protein microarray containing 2,724 Salmonella enterica serovar Typhi antigens (>63% of proteome) and identified antibodies against 16 IgG antigens and 77 IgM antigens that were differentially reactive among acute typhoid patients and healthy controls. The IgG target antigens produced a sensitivity of 97% and… 
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85 Citations
Highly Influential Citations
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Background Citations
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Methods Citations
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Results Citations
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85 Citations

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  • J. Andrews, F. Khanam, R. Charles
  • Medicine, Biology
    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
  • 2019
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A parsimonious serology signature to distinguish acute typhoid cases from controls is identified and validated in an independent test cohort from Nepal of patients with culture-confirmed S. Typhi and controls with other bacteremic illnesses.
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It is suggested that purified antigens could be used with ALS and corresponding acute-phase activated B lymphocytes in diagnostic platforms to identify acutely infected patients, even in areas where enteric fever is endemic.
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A Pneumococcal Protein Array as a Platform to Discover Serodiagnostic Antigens Against Infection*
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A protein microarray approach is taken to profile the humoral immune response to numerous infectious agents, and to identify the complete antibody repertoire associated with each disease, to provide a new understanding of the correlations between specific causative agents, the host response, and the clinical manifestations of the disease.
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