Immune profiling with a Salmonella Typhi antigen microarray identifies new diagnostic biomarkers of human typhoid

@article{Liang2013ImmunePW,
  title={Immune profiling with a Salmonella Typhi antigen microarray identifies new diagnostic biomarkers of human typhoid},
  author={Li Liang and Silvia Ju{\'a}rez and Tran Vu Thieu Nga and Sarah J. Dunstan and Rie Nakajima-Sasaki and D. Huw Davies and Stephen J. McSorley and Stephen Baker and Philip L. Felgner},
  journal={Scientific Reports},
  year={2013},
  volume={3}
}
Current serological diagnostic assays for typhoid fever are based on detecting antibodies against Salmonella LPS or flagellum, resulting in a high false-positive rate. Here we used a protein microarray containing 2,724 Salmonella enterica serovar Typhi antigens (>63% of proteome) and identified antibodies against 16 IgG antigens and 77 IgM antigens that were differentially reactive among acute typhoid patients and healthy controls. The IgG target antigens produced a sensitivity of 97% and… 
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