Corpus ID: 243855

Immune-complex phagocytosis by human polymorphonuclear granulocytes.

  title={Immune-complex phagocytosis by human polymorphonuclear granulocytes.},
  author={L{\'a}szl{\'o} Moln{\'a}r and J M{\'a}ty{\'a}s Bal{\'o}-Banga and {\'A}d{\'a}m Horv{\'a}th and J{\'a}nos Leibinger and E. Ablonczy and M. Nov{\'a}ki},
  journal={Acta physiologica Academiae Scientiarum Hungaricae},
  volume={52 1},
A new two-step centrifugation technique is described for the rapid preparation of viable pure polymorphonuclear granulocytes from 9.5 ml of venous blood. The phagocytosis of these cells was stimulated by soluble DNA-anti-DNA complexes, produced in vitro by incubating high mol. wt. DNA with sera obtained from patients with severe SLE. The measure of phagocytosis was the amount of nitroblue-tetrazolium (NBT), reduced by the active microphages. The reduced NBT was estimated by counting the… Expand
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