Immune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube (CPT™) and standard density gradient

@inproceedings{Corkum2015ImmuneCS,
  title={Immune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube (CPT™) and standard density gradient},
  author={Christopher P. Corkum and Danielle P. Ings and Christopher Burgess and Sylwia Karwowska and Werner Kroll and Tomasz I. Michalak},
  booktitle={BMC Immunology},
  year={2015}
}
High quality genetic material is an essential pre-requisite when analyzing gene expression using microarray technology. Peripheral blood mononuclear cells (PBMC) are frequently used for genomic analyses, but several factors can affect the integrity of nucleic acids prior to their extraction, including the methods of PBMC collection and isolation. Due to the lack of the relevant data published, we compared the Ficoll-Paque density gradient centrifugation and BD Vacutainer cell preparation tube… CONTINUE READING

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