Immortalized goat milk epithelial cell lines replicate CAEV at high level.

@article{MselliLakhal2001ImmortalizedGM,
  title={Immortalized goat milk epithelial cell lines replicate CAEV at high level.},
  author={Laila Mselli-Lakhal and François Guiguen and Claudie Fornazero and Colette Favier and Jitka Durand and D{\'e}lphine Grezel and Ahmed Moussa and Jean François Mornex and Yahia Chebloune},
  journal={Veterinary research},
  year={2001},
  volume={32 5},
  pages={429-40}
}
Primary milk epithelial cells were isolated from CAEV-uninfected goats and three cell lines designated TIGMEC-1, TIGMEC-2 and TIGMEC-3 were established. The three cell lines retained their morphological characteristics of epithelial cells and expressed specific epithelial cytokeratin marker as well as the immortalizing SV40 large T antigen. The kinetics of growth of TIGMEC1, TIGMEC2 and TIGMEC3 cell lines showed a doubling time of 24-48 hours while the parental cell lines became senescent after… CONTINUE READING
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