Immortalized dendritic cell line with efficient cross-priming ability established from transgenic mice harboring the temperature-sensitive SV40 large T-antigen gene.

@article{Ebihara2004ImmortalizedDC,
  title={Immortalized dendritic cell line with efficient cross-priming ability established from transgenic mice harboring the temperature-sensitive SV40 large T-antigen gene.},
  author={Shin Ebihara and Shota Endo and Kozue Ito and Yumi Ito and Kenichi Akiyama and Masuo Obinata and Toshiyuki Takai},
  journal={Journal of biochemistry},
  year={2004},
  volume={136 3},
  pages={321-8}
}
The uptake of an antigen and its presentation to specific T cells by dendritic cells (DCs) is a primary event in initiation of humoral and cellular immune responses as well as the induction of cytotoxic T cells (CTLs). DCs are induced by culturing bone marrow cells in the presence of GM-CSF. However, the resulting DCs are short-lived and the culture usually contains CD11c-negative non-DC cells, which adversely affects reproducibility and makes interpretation of the experimental results… CONTINUE READING