Immortalization of oral keratinocytes by functional inactivation of the p53 and pRb pathways

@article{Smeets2011ImmortalizationOO,
  title={Immortalization of oral keratinocytes by functional inactivation of the p53 and pRb pathways},
  author={Serge J. Smeets and Marlon van der Plas and Tieneke B.M. Schaaij-Visser and Eva A M van Veen and Johan van Meerloo and Boudewijn J. M. Braakhuis and Renske D. M. Steenbergen and Ruud H. Brakenhoff},
  journal={International Journal of Cancer},
  year={2011},
  volume={128}
}
A subgroup of head and neck squamous cell carcinomas (HNSCCs) contains high‐risk human papillomavirus‐type 16 (HPV16). The viral E6 and E7 oncoproteins inactivate the p53 and pRb proteins, respectively. We examined the causative effect of HPV16 E6 and E7 expression on the immortalization of normal oral keratinocytes (OKCs) and compared the resulting phenotype with alternative ways of p53‐ and pRb‐pathway abrogation frequently found in HNSCCs without HPV. Primary OKCs were conditionally… 

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This chapter summarizes the possible cell pathways involved in the activation of Wnt signaling in HPV-positive head and neck cancer.

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The generation of an HPV16 E6/E7 construct is described, which contains mutations that render E6-E7 non-oncogenic, while preserving antigenicity, and which statistically enhances clearance of established HPV(+) cancer in vivo.

The role of human papillomavirus in p16‐positive oral cancers

  • Simone BelobrovA. Cornall M. McCullough
  • Biology, Medicine
    Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
  • 2018
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A high prevalence of HPV16 as a primary HR-HPV type in LSCC and HPSCC is suggested and the lack of HPV E6/E7 oncoproteins in some tumor samples may suggest either the absence of viral integration or the presence of other mechanisms of tumorigenesis.
...

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