Immortalization of human preadipocytes.

  title={Immortalization of human preadipocytes.},
  author={Christian Darimont and Katherine Mac{\'e}},
  volume={85 12},
Primary human preadipocytes in culture are characterized by a low proliferative capacity associated with a rapid decline of differentiation ability during subculturing; thereby limiting their use as cellular model. Cellular immortalization constitutes an interesting approach for establishing cell lines presenting an unlimited life span and a maintained differentiation capacity. Different procedures for developing immortalized human preadipocytes are discussed in this review. Transformation of… CONTINUE READING